亚洲精品专区,四虎影视av,日本视频免费看

▍最近瀏覽歷史

[清空]

▍更多產(chǎn)品

貨號(hào)：B245025
名稱：Acanthamoeba healyi Moura et al.

貨號(hào)：B245024
名稱：Saccharomyces cerevisiae Meyen ex E.C. Hansen

貨號(hào)：B245023
名稱：Saccharomyces cerevisiae Meyen ex E.C. Hansen

貨號(hào)：B245022
名稱：Eutypella vitis (Schweinitz) Cooke emend. Glawe

貨號(hào)：B245021
名稱：Streptomyces scabiei (Thaxter) Lambert and Loria

貨號(hào)：B245020
名稱：Graphium cuneiferum (Berkeley et Broome) Mason et Ellis

貨號(hào)：B245019
名稱：HFBCI28

貨號(hào)：B245018
名稱：Septoria passiflorae Sydow, anamorph

貨號(hào)：B245017
名稱：Dictyostelium purpureum Olive

貨號(hào)：B245016
名稱：Y71

▍聯(lián)系我們

0574-87157013
mingzhoubio@163.com
浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
創(chuàng)e慧谷42號(hào)樓B幢401室

CRE BAG 2

規(guī)格:

貨期:

編號(hào):B162306

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

加入購(gòu)物車

在線咨詢收藏

產(chǎn)品名稱	CRE BAG 2
商品貨號(hào)	B162306
Organism	Mus musculus, mouse
Tissue	embryo
Product Format	frozen
Morphology	fibroblast
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	Leukemia
Strain	NIH/Swiss
Applications	produces protein beta-galactosidase beta-D-galactosidase
Derivation	The CRE BAG 2 cell line has never produced a replication competent helper virus. The cells will package vectors derived from murine leukemia viruses.
Genes Expressed	a beta galactosidase transducing vector (BAG),beta-galactosidase(beta-D-galactosidase) [lacZ],beta-galactosidase
Cellular Products	a beta galactosidase transducing vector (BAG)
Comments	The BAG construct has been used to transfect psiCRE cells in ATCC CRL-1858 and to transfect psi2 cells in ATCC CRL-9560. The CRE BAG 2 cell line has never produced a replication competent helper virus. This line produces a vector (BAG) that can infect mouse and rat cells and transduce beta-galactosidase activity. The line is similar to the psi 2 BAG alpha line (see ATCC CRL-9560) but has never been known to produce a replication competent helper virus. The cells will package vectors derived from murine leukemia viruses. The vector has been used to infect a variety of neural cell types and to express beta-galactosidase and resistance to G418.
Complete Growth Medium	Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; bovine calf serum, 10%
Subculturing	Medium Renewal: Every 2 to 3 days Remove medium, add fresh 0.25% trypsin - 0.02% EDTA and allow the flask to sit at room temperature (or 37C) until the cells detach (2 to 3 minutes). Add fresh medium, aspirate and dispense into new flasks.
Culture Conditions	Temperature: 37.0°C
Name of Depositor	CL Cepko
References	Price J, et al. Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer. Proc. Natl. Acad. Sci. USA 84: 156-160, 1987. PubMed: 3099292 Turner DL, Cepko CL. A common progenitor for neurons and glia persists in rat retina late in development. Nature 328: 131-136, 1987. PubMed: 3600789 Danos O, Mulligan RC. Safe and efficient generation of recombinant retroviruses with amphotropic and ecotropic host ranges. Proc. Natl. Acad. Sci. USA 85: 6460-6464, 1988. PubMed: 3413107 Cepko CLVirus propagation and Xgal proceduresIn: Cepko CLNeuromethodsClifton, NJHumana Press1989. Le Doux JM, et al. Proteoglycans secreted by packaging cell lines inhibit retrovirus infection. J. Virol. 70: 6468-6473, 1996. PubMed: 8709284

序號(hào)	文章標(biāo)題	操作
1	ATCC細(xì)胞運(yùn)輸和處理	查看詳情
2	其他海外品牌代理	查看詳情
3	ATCC代理	查看詳情
4	購(gòu)買須知	查看詳情
5	訂購(gòu)流程	查看詳情

梅經(jīng)理	17280875617	1438578920
胡經(jīng)理	13345964880	2438244627
周經(jīng)理	17757487661	1296385441
于經(jīng)理	18067160830	2088210172
沈經(jīng)理	19548299266	2662369050
李經(jīng)理	13626845108	972239479