| 產(chǎn)品名稱(chēng) |
Human rhinovirus 19 |
| 商品貨號(hào) |
B162804 |
| Classification |
Picornaviridae, Enterovirus, Rhinovirus A |
| Agent |
Human rhinovirus 19 |
| Strain |
6072 [6072-CV 19] |
| Applications |
Respiratory research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen 1.0 mL per vial |
| Storage Conditions |
-70°C or colder |
| Comments |
ATCC® 1929™ was derived from ATCC® 494™ following treatment with diethyl ether to remove Mycoplasma to remove mycoplasma contamination. |
| Effect on Host |
CPE, rounding and sloughing |
| Recommended Host |
EMEM (ATCC® 30-2003™) + 2% FBS (ATCC® 30-2020™) |
| Growth Conditions |
Temperature: 33.5°C
Recommendations for Infection: Plate cells 16-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 33.5°C in a humidified 5% CO2 atmosphere, rocking continuously. End adsorption by adding virus growth medium.
Incubation: 2-4 days at 33.5°C in a humidified 5% CO2 atmosphere, rocking continuously, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor |
VV Hamparian, RM Conant |
| Source |
Human throat washings |
| References |
Ketler A, et al. Characterization and classification of ECHO 28-rhinovirus-coryzavirus agents. Proc. Soc. Exp. Biol. Med. 110: 821-831, 1962. PubMed: 14455433
Palmerberg AC and Gern JE. Classification and evolution of human rhinoviruses. Methods Mol Biol. 1221:1-10, 2015. doi: 10.1007/978-1-4939-1571-2_1. PubMed: 25261302
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