| 產(chǎn)品名稱 | Bovine respiratory syncytial virus |
|---|---|
| 商品貨號 | B162837 |
| Classification | Pneumoviridae, Orthopneumovirus |
| Agent | Bovine respiratory syncytial virus |
| Strain | A 51908 |
| Applications | Respiratory research |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen |
| Storage Conditions | -70°C or colder |
| Images |  |
| Comments | ATCC® VR-1861™ was derived from ATCC® VR-794™ following treatment with Mycoplasma Removal Agent (MRA) to remove Mycoplasma contamination. |
| Effect on Host | CPE, Syncytia with cell sloughing/detachment |
| Recommended Host | BT cells (ATCC® CRL-1390™) |
| Growth Conditions | Temperature: 37°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 4-6 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80-90% of the monolayer. |
| Name of Depositor | SB Mohanty |
| Source | Trachea of calf with respiratory disease |
| References | Mohanty SB, et al. Experimentally induced respiratory syncytial viral infection in calves. Am. J. Vet. Res. 36: 417-419, 1975. PubMed: 164807 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
