| 產(chǎn)品名稱 |
Human coronavirus 229E |
| 商品貨號(hào) |
B163009 |
| Classification |
Coronaviridae, Coronavirus |
| Agent |
Human coronavirus 229E |
| Strain |
229E |
| Common Name |
Human coronavirus |
| Applications |
No hemolysis production. Respiratory research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen 1 mL per vial |
| Storage Conditions |
-70°C or colder |
| Comments |
Possibility of cross-reactions with other coronaviruses not thoroughly characterized at time of deposit (1973); does not cross-react with human myxoviruses. Ether sensitive. No hemolysis production. Unstable at 24 & 37C; relatively stable at 10C. Harvest 26-50 hrs post adsorption before CPE is visible. Does not agglutinate chicken, guinea pig, human O, sheep, mouse or rat RBC. Size 89Mu (gradocol filtration). [RU1 are human embryonic lung fibroblasts developed by CDC] |
| Effect on Host |
CPE, rounding and sloughing. Starts as individual cell rounding that spreads, but over time uninfected cells repopulate monolayer. |
| Recommended Host |
MRC-5 (ATCC® CCL-171™) |
| Growth Conditions |
Temperature: 35°C
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent).
Incubation: 2-7 days, a 5% CO2 in air atmosphere is recommended. |
| Name of Depositor |
HS Kaye |
| Special Collection |
ATCC |
| Source |
Nasal and throat swabs from man with mild upper respiratory illness |
| References |
Hamre D, Procknow JJ. A new virus isolated from the human respiratory tract. Proc. Soc. Exp. Biol. Med. 121: 190-193, 1966. PubMed: 4285768
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