| 產(chǎn)品名稱 |
Bovine adenovirus 4 |
| 商品貨號(hào) |
B163431 |
| Classification |
Adenoviridae, Atadenovirus, |
| Deposited As |
Bovine adenovirus type 4 |
| Agent |
Bovine adenovirus 4 |
| Strain |
THT/62 |
| Applications |
Respiratory research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
Lungs of a calf with pneumoenteritis |
| Storage Conditions |
?-70°C or colder |
| Comments |
Does not share common CF antigen with human adenoviruses. No cross-reactivity with bovine adenovirus types 1-3. GenBank: AF036092.3 |
| Effect on Host |
CPE, generalized enlarged cell rounding and cell clumping or clustering; Type B intranuclear inclusion bodies ?CPE, generalized enlarged cell rounding and cell clumping or clustering; Type B intranuclear inclusion bodies |
| Recommended Host |
BT (ATCC CRL-1390) Production Host: ?BT (ATCC? CRL-1390™) |
| Growth Conditions |
Temperature: 37.0°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume (e.g. 1 mL per 25 cm2) of virus diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C without CO2, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: Incubate infected culture for 6-7 days at 37°C without CO2, until 90% of the culture shows CPE. |
| Name of Depositor |
SB Mohanty |
| Chain of Custody |
Deposited as Bovine adenovirus type 4. Originally received after 6 passages in primary calf testicular cells. |
| Source |
Lungs of a calf with pneumoenteritis |
| References |
Bartha A, Aldasy P. Further two serotypes of bovine adenoviruses (serotype 4 and 5). Acta Vet. Acad. Sci. Hung. 16(1): 107-108, 1966. PubMed: 5917816
|
| Cross References |
GenBank :
AF036092.3
Bovine adenovirus 4 strain THT/62, complete genome.
|
