| 產(chǎn)品名稱(chēng) |
10-1.D.2 |
| 商品貨號(hào) |
B163532 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody induces blastogenesis in B cells, and this response is limited to the Lyb 5+ class of B cells. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against Lyb 2.1 mouse B cell (B lymphocyte) antigen |
| Cellular Products |
immunoglobulin; monoclonal antibody; against Lyb 2.1 mouse B cell (B lymphocyte) antigen |
| Comments |
Animals were immunized with spleen cells from DBA/2 mice. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. Lyb-2.1 antigen is a mouse B cell differentiation antigen (45000 dalton glycoprotein). The antibody induces blastogenesis in B cells, and this response is limited to the Lyb 5+ class of B cells. B cells activated in this way mature into immunoglobulin secreting cells in the presence of T cell replacing factors (medium from Con A treated T cells). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
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| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype |
IgG2b |
| Name of Depositor |
DE Mosier |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Subbarao B, Mosier DE. Induction of B lymphocyte proliferation by monoclonal anti-Lyb 2 antibody. J. Immunol. 130: 2033-2037, 1983. PubMed: 6601145
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