| 產(chǎn)品名稱 |
12.1 |
| 商品貨號(hào) |
B163549 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
sarcoma |
| Applications |
The antibody strongly reacts with an antigen (CD6) expressed on peripheral blood T cells, activated T cells and Sezary type leukemic T cells. Lesser amounts of the antigen were detected on thymocytes and acute T cell leukemic lympoblasts. The antibody also reacts with leukemic cells from most patients with B cell chronic lymphocytic leukemia and some patients with lymphosarcoma cell leukemia. |
| Derivation |
Animals were immunized with human lymphoblasts from a mixed lymphocyte culture reaction. Spleen cells were fused with NSI/1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human CD6 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human CD6 |
| Comments |
The antibody strongly reacts with an antigen (CD6) expressed on peripheral blood T cells, activated T cells and Sezary type leukemic T cells. Lesser amounts of the antigen were detected on thymocytes and acute T cell leukemic lympoblasts. The antibody also reacts with leukemic cells from most patients with B cell chronic lymphocytic leukemia and some patients with lymphosarcoma cell leukemia. |
| Complete Growth Medium |
The base medium for this cell line is RPMI 1640 Medium (ATCC 30-2001). To make the complete medium add the following components to 500 mL of the base medium:
- 2-mercaptoethanol for a final concentration of 50 µM
- 56 mL Fetal Bovine Serum (FBS; ATCC 30-2020) for a final concentration of 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml. |
| Isotype |
IgG2a |
| Name of Depositor |
PJ Martin |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Kamoun M, et al. A novel human T cell antigen preferentially expressed on mature T cells and shared by both well and poorly differentiated B cell leukemias and lymphomas. J. Immunol. 127: 987-991, 1981. PubMed: 6790625
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