| 產(chǎn)品名稱 |
17D |
| 商品貨號(hào) |
B163604 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody is specific for sheep CD4 and precipitates a 56000 dalton molecule from sheep T cell lysates. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against ovine (sheep) CD4 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against ovine (sheep) CD4 |
| Comments |
Animals were immunized with phytohemagglutinin activated sheep T cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is specific for sheep CD4 and precipitates a 56000 dalton molecule from sheep T cell lysates. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) cells/ml.
Interval: Maintain cultures at a cell concentration between 1 X 10(5) and 1 X 10(6) cells/ml Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype |
mouse IgG1 |
| Name of Depositor |
CR Mackay |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Mackay CR, et al. Unusual expression of CD2 in sheep: implications for T cell interactions. Eur. J. Immunol. 18: 1681-1688, 1988. PubMed: 2462499
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