| 產(chǎn)品名稱 |
1C3 |
| 商品貨號 |
B163618 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The cell line was established by Jose A. Lebron and Pamela J. Bjorkman in 1997. Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]). This antibody can be used for ELISA applications and to immunoprecipitate soluble HFE; it is not reactive in Western Blot assays. |
| Derivation |
The cell line was established by Jose A. Lebron and Pamela J. Bjorkman in 1997. Animals were tolerized with human beta2-microglobulin and immunized with purified, soluble, human HFE. Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]). |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against HFE |
| Cellular Products |
immunoglobulin; monoclonal antibody; against HFE |
| Comments |
The cell line was established by Jose A. Lebron and Pamela J. Bjorkman in 1997. Animals were tolerized with human beta2-microglobulin and immunized with purified, soluble, human HFE. Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]). The antibody recognizes the HFE portion of the HFE/beta2 heterodimer. HFE is a major histocompatibility complex (MHC) related protein that is mutated in the iron-overload disease hereditary hemochromatosis. This antibody can be used for ELISA applications and to immunoprecipitate soluble HFE; it is not reactive in Western Blot assays. |
| Complete Growth Medium |
HL-1 medium supplemented with 4 mM L-glutamine, 1 mM sodium pyruvate and 1% fetal bovine serum. HL-1 medium can be obtained from from Lonza (catalog number 77201).
|
| Subculturing |
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. Attached cells may be subcultured by tapping the sides of the flask until cells are dispersed. |
| Cryopreservation |
HL-1 Complete Serum-free Medium, 75%; fetal bovine serum, 20%; DMSO, 5%. |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
PJ Bjorkman |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| Year of Origin |
1997 |
| References |
Lebron JA, et al. Tolerization of adult mice to immunodominant proteins before monoclonal antibody production. J. Immunol. Methods 222: 59-63, 1999. PubMed: 10022372
Lebron JA, et al. Crystal structure of the hemochromatosis protein HFE and characterization of its interaction with transferrin receptor. Cell 93: 111-123, 1998. PubMed: 9546397
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