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• O.1 mM Non-Essential Amino Acids (NEAA)
• fetal bovine serum to a final concentration of 10%

Temperature: 37°C
Atmosphere: 5% CO₂

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Dasch JR, et al. Monoclonal antibodies to transforming growth factor-beta and methods of use. US Patent 5,783,185 dated Jul 21 1998

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Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.