| 產(chǎn)品名稱 |
25-9-3S |
| 商品貨號 |
B163651 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against I-A b; cytotoxic |
| Cellular Products |
immunoglobulin; monoclonal antibody; against I-A b; cytotoxic |
| Comments |
Animals were immunized with spleen cells from C3H.SW mice. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. No cross-reactions are known. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Culture Medium: Modified Dulbecco's medium (see below), 90%; fetal bovine
serum, 10%.
DME w/HEPES (10mM) 100 mL
L-Glutamine (100x) 1 mL
Solution I 1 mL
Non-essential amino acids (100x) 1 mL
NCTC 135 10 mL
Fetal Bovine Serum 12 mL
Solution I (100x)
1. 1320 mg oxalacetic acid (100 mM, MW 132).
2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to
100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
aliquot and store frozen. |
| Subculturing |
Medium Renewal: Every 1 to 2 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Freeze medium: complete growth medium, 95% DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Isotype |
IgM |
| Name of Depositor |
DH Sachs, K Ozato |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J. Immunol. 126: 317-321, 1981. PubMed: 6935293
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