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Cultures can be maintained by addition of fresh medium or by replacement of medium. Adherent cells can be dislodged by scraping, and cultures may be established by centrifugation with subsequent resuspension at 2 x 10⁵ viable cells/mL. A saturation density of 1 x 10⁶ cells/mL is obtainable.

Temperature: 37°C
Atmosphere: 5% CO₂

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