| 產品名稱 |
3C5.1 |
| 商品貨號 |
B163732 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with the 220 kD catalytic subunit of DNA polymerase epsilon. The 3C5.1 hybridoma was developed in 1993 by Gloria Chui, and can be used in Western immunoblot techniques to detect the presence of DNA polymerase epsilon. |
| Derivation |
Animals were immunized with Mono S FPLC purified DNA polymerase epsilon. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The 3C5.1 hybridoma was developed in 1993 by Gloria Chui, and can be used in Western immunoblot techniques to detect the presence of DNA polymerase epsilon. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human DNA polymerase epsilon (pol epsilon) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human DNA polymerase epsilon (pol epsilon) |
| Comments |
Animals were immunized with Mono S FPLC purified DNA polymerase epsilon. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with the 220 kD catalytic subunit of DNA polymerase epsilon. It does not recognize DNA polymerases alpha, beta or delta. The 3C5.1 hybridoma was developed in 1993 by Gloria Chui, and can be used in Western immunoblot techniques to detect the presence of DNA polymerase epsilon. |
| Complete Growth Medium |
Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM hypoxanthine and 0.008 mM thymidine, 80%; fetal bovine serum, 20%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2a |
| Name of Depositor |
S Linn |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Chui G, Linn S. Further characterization of HeLa DNA polymerase epsilon. J. Biol. Chem. 270: 7799-7808, 1995. PubMed: 7536191
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