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4E11

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編號:B163768

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產(chǎn)品名稱	4E11
商品貨號	B163768
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Storage Conditions	liquid nitrogen vapor phase
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation	Animals were immunized with DYKDDDDK. Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against a synthetic peptide Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (DYKDDDDK)
Cellular Products	immunoglobulin; monoclonal antibody; against a synthetic peptide Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (DYKDDDDK)
Tumorigenic	Yes
Effects	Forms ascites in pristane primed BALB/c mice.
Comments	The antibody reaction with the peptide is calcium dependent and the antibody can be used to isolate fusion proteins tagged with the DYKDDDDK peptide. The resulting antigen - antibody complex can be dissociated by removal of Ca++ from the buffer.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing	Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10⁵ viable cells/mL. Maintain cultures at a cell concentration between 1 x 10⁵ and 1 x 10⁶ cells/mL. Medium Renewal: Every 2 to 3 days
Cryopreservation	Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Isotype	IgG2b
Name of Depositor	Immunex Corporation
Deposited As	mouse (B cell); mouse (myeloma)
U.S. Patent Number	4,851,341
References	Hopp TP, Prickett KS. Immunoaffinity purification system. US Patent 4,851,341 dated Jul 25 1989 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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