| 產品名稱 |
5/9 m alpha3-18 |
| 商品貨號 |
B163773 |
| Organism |
Cricetulus griseus, hamster, Chinese |
| Tissue |
ovary |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender |
female |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
This line was derived from the CHO DUKX cell line. |
| Clinical Data |
female |
| Genes Expressed |
human colony stimulating factor (M-CSF) |
| Cellular Products |
human colony stimulating factor (M-CSF) |
| Comments |
The cells are resistant to methotrexate. |
| Complete Growth Medium |
Alpha minimum essential medium with 2 mM L-glutamine and 1 mM sodium pyruvate without ribonucleosides and deoxyribonucleosides supplemented with 200 nM MTX (Methotrexate) and 10% dialyzed fetal bovine serum (Sigma F0392) |
| Subculturing |
- Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.
Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
-
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:20 is recommended
Medium Renewal: Two to three times weely |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor |
Genetics Institute, Inc. |
| Deposited As |
Cricetulus griseus |
| U.S. Patent Number |
|
| References |
Clark SC, Wong GG. Hematopoietic growth factors. US Patent 4,868,119 dated Sep 19 1989
|
