| 產(chǎn)品名稱 |
520C9 [520C9.C3B10T] |
| 商品貨號(hào) |
B163777 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Cancer,Cancer |
| Applications |
By immunofluorescence, the antibody is specific for neoplastic breast tissue with some weak cross-reaction with other neoplasms. The antibody reacts with a 210000 dalton protein found in cancerous human breast tissue. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human breast cancer cells |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human breast cancer cells |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in BALB/c mice |
| Comments |
Animals were immunized either with live cells or with membrane extracts from human breast cancer cell lines. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. By immunofluorescence, the antibody is specific for neoplastic breast tissue with some weak cross-reaction with other neoplasms. The antibody reacts with a 210000 dalton protein found in cancerous human breast tissue. |
| Complete Growth Medium |
Culture Medium: Dulbecco's modified Eagle's medium (containing: 0.2 units/mL
insulin, 1 mM Na pyruvate, 0.15 mg/mL oxaloacetic acid), with 4.5 g/L glucose,
60%; NCTC 109 medium, 20%; fetal bovine serum, 20%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
Cetus Corp. |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Ring DB, et al. Monoclonal anti-human breast cancer antibodies. US Patent 4,753,894 dated Jun 28 1988
Tempest PR, et al. Humanized antibodies to Fc receptors for immunoglobulin G on human mononuclear phagocytes. US Patent 6,500,931 dated Dec 31 2002
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