| 產品名稱 |
60bca |
| 商品貨號 |
B163799 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with human CD14, inhibits the binding of LPS - LPS binding protein complexes to CD14 on human phagocytes and prevents the release of tumor necrosis factor alpha. |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human CD14 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human CD14 |
| Comments |
Animals were immunized with cultured human peripheral blood monocytes. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with human CD14, inhibits the binding of LPS - LPS binding protein complexes to CD14 on human phagocytes and prevents the release of tumor necrosis factor alpha. |
| Complete Growth Medium |
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium: - heat-inactivated gamma globulin free horse serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
RF Todd |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Ashmun RA, et al. Expression of the human monocyte membrane antigen gp55 by murine fibroblasts after DNA-mediated gene transfer. Blood 69: 886-892, 1987. PubMed: 3545322
Wright SD, et al. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science 249: 1431-1433, 1990. PubMed: 1698311
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