| 產品名稱 |
63D3 |
| 商品貨號 |
B163801 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with a 200000 dalton molecule on human monocytes. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Animals were immunized with purified human peripheral blood monocytes. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human monocyte |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human monocyte |
| Comments |
Animals were immunized with purified human peripheral blood monocytes. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with a 200000 dalton molecule on human monocytes. It is not cytotoxic and does not react with human B cells, T cells or endothelial cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;
fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
JD Capra |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Ugolini V, et al. Initial characterization of monoclonal antibodies against human monocytes. Proc. Natl. Acad. Sci. USA 77: 6764-6768, 1980. PubMed: 6935683
|
