| 產品名稱 |
68-1-2 |
| 商品貨號 |
B163803 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody is specific for the alpha subunit and recognizes a determinant present only on the denatured enzyme. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Mice were immunized with E. coli DNA polymerase III' containing the alpha, epsilon, theta and tau subunits. Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody is specific for the alpha subunit and recognizes a determinant present only on the denatured enzyme. The site recognized by the 68-1-2 antibody is distinct from that recognized by 123-10 (ATCC CRL-1707) but is adjacent to, or interacts with, the 123-10 site. |
| Comments |
Mice were immunized with E. coli DNA polymerase III' containing the alpha, epsilon, theta and tau subunits. Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody is specific for the alpha subunit and recognizes a determinant present only on the denatured enzyme. The site recognized by the 68-1-2 antibody is distinct from that recognized by 123-10 (ATCC CRL-1707) but is adjacent to, or interacts with, the 123-10 site. |
| Complete Growth Medium |
Iscove's modified Dulbecco's medium, 85%; fetal bovine serum, 15%
|
| Subculturing |
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype |
IgM |
| Name of Depositor |
M Franden, CS McHenry |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Wu YH, et al. Monoclonal antibodies specific for the alpha subunit of the Escherichia coli DNA polymerase III holoenzyme. J. Biol. Chem. 259: 12117-12122, 1984. PubMed: 6090455
The determinant is present only on the denatured enzyme
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