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產品名稱	90196B
商品貨號	B163848
Organism	Homo sapiens, human
Tissue	spleen
Product Format	frozen
Morphology	monocyte/macrophage
Culture Properties	suspension
Biosafety Level	2 [Cells contain Epstein-Barr DNA viral sequences] Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Storage Conditions	liquid nitrogen vapor phase
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation	The 90196B cell line was established from cryopreserved human spleen cells.
Comments	The cells tend to grow in floating clusters. Single cells may become adherent.
Complete Growth Medium	Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
Subculturing	The cells tend to grow in floating clusters. Single cells may become adherent. Cultures can be maintained by addition or replacement of fresh medium. Maintain the cell concentration between 2 x 10⁵ and 1x10⁶cells/mL. Gently pipette to break large clusters into smaller clusters. Medium Renewal: Twice weekly
Cryopreservation	Culture medium,85%; DMSO,10%; extra FBS,10%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C
Name of Depositor	E.I. du Pont de Nemours & Co.
Deposited As	Homo sapiens
U.S. Patent Number	5,447,861
References	Collins GW, Largen MT. Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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