| 產品名稱 |
9D9 |
| 商品貨號 |
B163856 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with LDL receptors of normal rabbits but not with those of Watanabe Heritable Hyperlipidemic rabbits. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Antigen Expression |
H-2d |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against bovine low density lipoprotein (LDL) receptor,H-2d |
| Cellular Products |
immunoglobulin; monoclonal antibody; against bovine low density lipoprotein (LDL) receptor |
| Comments |
Mice were immunized with trypsin digested bovine adrenal LDL receptor. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with LDL receptors of normal rabbits but not with those of Watanabe Heritable Hyperlipidemic rabbits. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Maintain cell concentration between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
JL Goldstein |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Huettinger M, et al. Use of monoclonal anti-receptor antibodies to probe the expression of the low density lipoprotein receptor in tissues of normal and Watanabe Heritable Hyperlipidemic rabbits. J. Clin. Invest. 74: 1017-1026, 1984. PubMed: 6088578
Kita T, et al. Deficiency of low density lipoprotein receptors in liver and adrenal gland of the WHHL rabbit, an animal model of familial hypercholesterolemia. Proc. Natl. Acad. Sci. USA 78: 2268-2272, 1981. PubMed: 6264472
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