| 產(chǎn)品名稱 |
A-172 [A172] |
| 商品貨號(hào) |
B163865 |
| Organism |
Homo sapiens, human |
| Tissue |
brain |
| Product Format |
frozen |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
glioblastoma |
| Age |
53 years |
| Gender |
male |
| Storage Conditions |
liquid nitrogen vapor phase |
| Clinical Data |
male |
| Tumorigenic |
No |
| Effects |
Yes, form colonies in semisolid medium. No, the cells were not tumorigenic in immunosuppressed mice |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: culture medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 9,12 D13S317: 11 D16S539: 12 D5S818: 11,12 D7S820: 11 THO1: 6,9.3 TPOX: 8,11 vWA: 20 |
| Name of Depositor |
DJ Giard |
| Deposited As |
Homo sapiens |
| References |
Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221
Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758
Debinski W, et al. Receptor for interleukin (IL) 13 does not interact with IL4 but receptor for IL4 interacts with IL13 on human glioma cells. J. Biol. Chem. 271: 22428-22433, 1996. PubMed: 8798406
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