| 產(chǎn)品名稱 | A-253 |
|---|---|
| 商品貨號 | B163870 |
| Organism | Homo sapiens, human |
| Tissue | submaxillary salivary gland |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | epidermoid carcinoma |
| Age | 54 years adult |
| Gender | male |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | near triploid with at least 6 markers |
| Images |  |
| Derivation | The A253 line is one of thirteen to be isolated and partially characterized through trial studies on a series of 200 human tumors. |
| Clinical Data | 54 years adult Caucasian male |
| Tumorigenic | Yes |
| Effects | Yes, in nude mice (forms undifferentiated carcinoma) Yes, anti thymocyte serum treated newborn mice |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
|
| Cryopreservation | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile | Amelogenin: X CSF1PO: 12,13 D13S317: 9,12 D16S539: 12 D5S818: 11 D7S820: 10,12 THO1: 6,8 TPOX: 8,12 vWA: 16 |
| Isoenzymes | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 PGM1, 1 PGM3, 1 |
| Name of Depositor | G Todaro |
| Deposited As | Homo sapiens |
| References | Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216 Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
