| 產(chǎn)品名稱 | A-427 |
|---|---|
| 商品貨號 | B163881 |
| Organism | Homo sapiens, human |
| Tissue | lung |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | carcinoma |
| Age | 52 years |
| Gender | male |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | at passage 60, hypotriploid to hypertriploid with abnormalities including dicentrics, minutes and large subtelocentric marker |
| Images |  |
| Derivation | The A-427 line was derived by D.J. Giard, as indicated in the description for ATCC HTB-41. |
| Clinical Data | 52 years Caucasian male |
| Tumorigenic | Yes |
| Effects | Yes, in nude mice; forms an undifferentiated tumor suggestive of adenocarcinoma |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Interval: every 6 to 8 days
Subcultivation Ratio: 1:2 to 1:6
Medium Renewal: Twice per week |
| Cryopreservation | Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C |
| STR Profile | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 11,12 D16S539: 11,13 D5S818: 12 D7S820: 8,12 THO1: 9 TPOX: 8,11 vWA: 17 |
| Isoenzymes | AK-1, 2 ES-D, 1 G6PD, B GLO-I, 1 PGM1, 1-2 PGM3, 1 |
| Name of Depositor | DJ Giard |
| Deposited As | Homo sapiens |
| References | Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
