| 產(chǎn)品名稱 |
A431NS |
| 商品貨號(hào) |
B163883 |
| Organism |
Homo sapiens, human |
| Tissue |
skin/epidermis |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
epidermoid carcinoma |
| Age |
85 years |
| Gender |
female |
| Applications |
While cell sensitivity to drugs tend to be changeable, the A431NS cell line has a stable sensitivity to LIC. The cell line can be used to test the antiproliferation activity of LIC. |
| Storage Conditions |
liquid nitrogen vapor temperture |
| Derivation |
A431NS was derived from the A431 cell line (ATCC CRL-1555) in 1997 by repeated subculturing to select cells that detached from the substrate easily. |
| Clinical Data |
female
85 years |
| Comments |
The polyinosinic-polycytidylic acid/cationic liposome complex (LIC) inhibits cancer cell growth by the induction of apoptosis.
LIC has strong cytotoxic effects on the A431NS cell line but neither polyinosinic-polycytidylic acid alone nor cationic liposome alone have antiproliferation effects.
|
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with PBS to remove all traces of serum which contains trypsin inhibitor.
- Remove the PBS completely and add 2.0 to 3.0 ml of 0.25% (w/v) Trypsin-053mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually with 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium containing 10% FBS and aspirate cells by gently pipetting.
- To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes.
- Discard supernatant and resuspend cells in fresh culture medium. Add appropriate aliquots of cell suspension to new culture vessels.
- Place culture vessels in incubators at 37°C.
Subcultivation Ratio: 1:3 to 1:6.
Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994
|
| Cryopreservation |
Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperture |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 11,12 D13S317: 9,13 D16S539: 12,14 D5S818: 12,13 D7S820: 10 THO1: 9 TPOX: 11 vWA: 15,17 |
| Name of Depositor |
K Hirabayashi |
| Deposited As |
human |
| References |
Hirabayashi K, et al. Inhibition of cancer cell growth by polyinosinic-polycytidylic acid/cationic liposome complex: a new biological activity. Cancer Res. 59: 4325-4333, 1999. PubMed: 10485480
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