| 產品名稱 |
A6 [A-6] |
| 商品貨號 |
B163887 |
| Organism |
Homo sapiens; Mus musculus, human; mouse |
| Cell Type |
Hybridoma |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension, with some loosely adherent cells |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The line was produced by fusing a G-418 resistant derivative of the U-266 human myeloma cell line with P3X63Ag8.653 mouse myeloma cells. The cells are useful as fusion partners for human B cells in the generation of human hybridomas. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
The line was produced by fusing a G-418 resistant derivative of the U-266 human myeloma cell line with P3X63Ag8.653 mouse myeloma cells. |
| Comments |
The line was produced by fusing a G-418 resistant derivative of the U-266 human myeloma cell line with P3X63Ag8.653 mouse myeloma cells. G-418 resistance was introduced to the human myeloma by transfection with pSV-2 neo r. The cells are useful as fusion partners for human B cells in the generation of human hybridomas. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing |
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x10(5) viable cells/ml. Maintain cultures at a cell concentration between 1 x 10(5) and 1 x 10(6) cells/ml. Scrape attached cells if necessary. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: 75% CompleteGrowth Medium + 20% FBS + 5% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
H Kaplan |
| Deposited As |
human; mouse |
| U.S. Patent Number |
|
| References |
Kaplan HS, et al. Methods and cell lines for immortalization and monoclonal antibody production by antigen-stimulated B-lymphocytes. US Patent 4,574,116 dated Mar 4 1986
|
