| 產品名稱 |
A9 |
| 商品貨號 |
B163895 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Cell membranes used for immunization derived from UT-1, a cell line derived from the CHO K1 cell line.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
Immunoglobulin; monoclonal antibody; against HMG-CoA reductase (3-hydroxy-3-methyl-glutaryl Coenzyme A reductase, HMG CoA reductase) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against HMG-CoA reductase (3-hydroxy-3-methyl-glutaryl Coenzyme A reductase, HMG CoA reductase) |
| Tumorigenic |
Yes |
| Effects |
Yes, in pristane primed BALB/c mice |
| Comments |
Animals were immunized with cell membranes from UT-1.
The antibody reacts with HMG-CoA reductase (the rate limiting enzyme in the cholesterol biosynthetic pathway).
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Isotype |
IgG1 |
| Name of Depositor |
JL Goldstein, YK Ho |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Liscum L, et al. Regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase and its mRNA in rat liver as studied with a monoclonal antibody and a cDNA probe. J. Biol. Chem. 258: 8450-8455, 1983. PubMed: 6688077
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