| 產品名稱 |
ACT I |
| 商品貨號 |
B163906 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with actin from prokaryotic organisms at an epitope distinct from that recognized by ACT IV (ATCC HB-81). Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against actin |
| Cellular Products |
immunoglobulin; monoclonal antibody; against actin |
| Comments |
Animals were immunized with actin from Dictyostelium discoideum. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with actin from prokaryotic organisms at an epitope distinct from that recognized by ACT IV (ATCC HB-81). The antibody does not cross-react with eukaryotic actins. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
- fetal bovine serum to a final concentration of 20%
- HAT (13.6 mg/L hypoxanthine (0.1 mM), 0.176 mg/L aminopterin (0.4 μM) and 3.8 mg/L thymidine 0.016 mM)
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
P Parham |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Simpson PA, et al. Monoclonal antibodies prepared against Dictyostelium actin: characterization and interactions with actin. J. Cell Biol. 99: 287-295, 1984. PubMed: 6203918
|
