Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody reacts strongly with insulin receptors from human placenta and IM-9 cells (ATCC CCL-159).
It reacts less efficiently with insulin receptors on human erythrocytes.
The antibody cross-reacts with the receptor for somatomedin C.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with purified human placental insulin receptor.
Spleen cells were fused with FO myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human insulin receptor
Cellular Products
immunoglobulin; monoclonal antibody; against human insulin receptor
Comments
Animals were immunized with purified human placental insulin receptor.
Spleen cells were fused with FO myeloma cells.
The antibody reacts strongly with insulin receptors from human placenta and IM-9 cells (ATCC CCL-159).
It reacts less efficiently with insulin receptors on human erythrocytes.
The antibody cross-reacts with the receptor for somatomedin C.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1; kappa light chain
Name of Depositor
S Jacobs, FC Kull
Deposited As
mouse (B cell); mouse (myeloma)
References
Kull FC Jr., et al. Monoclonal antibodies to receptors for insulin and somatomedin-C. J. Biol. Chem. 258: 6561-6566, 1983. PubMed: 6304046
