| Applications |
The antibody reacts with the family of 6 essential splicing factors, the SR proteins. Specificity is similar to Mab104 (ATCC CRL-2067) but because it is an IgG may be potentially more useful than Mab104 which is an IgM. This antibody may be useful to determine whether a protein studied for its interactions with RNA is a SR protein and it is also useful in Western Blot, immunoprecipitation, and cytological assays. |
| Comments |
Animals were immunized with mass-isolated nuclei from the oocytes of Xenopus laevis. Spleen cells were fused with FOX-NY myeloma cells. The antibody reacts with the family of 6 essential splicing factors, the SR proteins. SR proteins are a family of proteins that have pre-mRNA splicing activity (SR comes from the sequences of consecutive serine and arginine residues in these proteins). The family consists of at least 6 proteins with approximate masses of 75000, 55000, 40000, two at 30000 and 20000 daltons. Specificity is similar to Mab104 (ATCC CRL-2067) but because it is an IgG may be potentially more useful than Mab104 which is an IgM. This antibody may be useful to determine whether a protein studied for its interactions with RNA is a SR protein and it is also useful in Western Blot, immunoprecipitation, and cytological assays. |
| References |
Neugebauer KM, Roth MB. Distribution of pre-mRNA splicing factors at sites of RNA polymerase II transcription. Genes Dev. 11: 1148-1159, 1997. PubMed: 9159396
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
|
