| Applications |
Animals were immunized with a peptide synthesized to correspond to amino acids 84 to 104 of the predicted protein that occur between the RNA recognition motif and the SR domain. The antibody reacts with a single member, SRp20, of the family of essential splicing factors the SR proteins. The antibody is used to determine if a factor in RNA metabolism is SRp20. The antibody is useful for immunoprecipitation, immunoblotting and immunocytochemistry assays |
| Comments |
Animals were immunized with a peptide synthesized to correspond to amino acids 84 to 104 of the predicted protein that occur between the RNA recognition motif and the SR domain. Spleen cells were fused with FOX-NY myeloma cells. The antibody reacts with a single member, SRp20, of the family of essential splicing factors the SR proteins. SR proteins are a family of proteins that have pre-mRNA splicing activity (SR comes from the sequences of consecutive serine and arginine residues in these proteins). The family consists of at least 6 proteins with approximate masses of 75000, 55000, 40000, two at 30000 and 20000 daltons. The antibody is used to determine if a factor in RNA metabolism is SRp20. The antibody is useful for immunoprecipitation, immunoblotting and immunocytochemistry assays |
| References |
Neugebauer KM, Roth MB. Distribution of pre-mRNA splicing factors at sites of RNA polymerase II transcription. Genes Dev. 11: 1148-1159, 1997. PubMed: 9159396
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
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