| Comments |
Animals were immunized with adaptor protein. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with the alpha chain of the AP-2 protein of all mammalian species tested. It is useful for immunofluorescence and immunoprecipitation assays, but tends to give spurious bands in Western blots. |
| References |
Chin DJ, et al. 100-kDa polypeptides in peripheral clathrin-coated vesicles are required for receptor-mediated endocytosis. Proc. Natl. Acad. Sci. USA 86: 9289-9293, 1989. PubMed: 2574457
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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