| 產品名稱 |
AsPC-1 |
| 商品貨號 |
B163960 |
| Organism |
Homo sapiens, human |
| Tissue |
pancreas; derived from metastatic site: ascites |
| Product Format |
frozen |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
adenocarcinoma |
| Age |
62 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Applications |
This cell line is a suitable transfection host. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
The line was derived from nude mouse xenografts initiated with cells from the ascites of a patient with cancer of the pancreas. |
| Clinical Data |
62 years
Caucasian
female
|
| Genes Expressed |
carcinoembryonic antigen (CEA); human pancreas associated antigen; human pancreas specific antigen; mucin |
| Cellular Products |
carcinoembryonic antigen (CEA); human pancreas associated antigen; human pancreas specific antigen; mucin |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
- Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
-
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 10,13 D13S317: 9,12 D16S539: 11 D5S818: 12 D7S820: 12,13 THO1: 7,9.3 TPOX: 8,10 vWA: 17 |
| Name of Depositor |
MH Tan |
| Deposited As |
Homo sapiens |
| References |
Chen WH, et al. Human pancreatic adenocarcinoma: in vitro and in vivo morphology of a new tumor line established from ascites. In Vitro 18: 24-34, 1982. PubMed: 7182348
Tan MH, et al. Differential localization of human pancreas cancer-associated antigen and carcinoembryonic antigen in homologous pancreatic tumoral xenograft. J. Natl. Cancer Inst. 67: 563-569, 1981. PubMed: 6944528
Loor R, et al. Use of pancreas-specific antigen in immunodiagnosis of pancreatic cancer. Clin. Lab. Med. 2: 567-578, 1982. PubMed: 6754226
Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903
Tan MH, Chu TM. Characterization of the tumorigenic and metastatic properties of a human pancreatic tumor cell line (AsPC-1) implanted orthotopically into nude mice. Tumor Biol. 6: 89-98, 1985. PubMed: 4023565
|
| Cross References |
Nucleotide (GenBank) :
AA640215
01 subtracted 3' EST library Homo sapiens cDNA clone U32, mRNA sequence.
Nucleotide (GenBank) :
AA640216
02 subtracted 3' EST library Homo sapiens cDNA clone U30, mRNA sequence.
Nucleotide (GenBank) :
AA640217
03 subtracted 3' EST library Homo sapiens cDNA clone D32, mRNA sequence.
Nucleotide (GenBank) :
AA640218
04 subtracted 3' EST library Homo sapiens cDNA clone D42, mRNA sequence.
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