| 產(chǎn)品名稱(chēng) |
B16-F1 |
| 商品貨號(hào) |
B163972 |
| Organism |
Mus musculus, mouse |
| Tissue |
skin |
| Product Format |
frozen |
| Morphology |
mixture of spindle-shaped and epithelial-like cells |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
melanoma |
| Strain |
C57BL/6J |
| Applications |
This cell line is a suitable transfection host. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Tumorigenic |
Yes |
| Effects |
Yes, in syngeneic mice |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:10 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: culture medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
