| 產品名稱 |
BB7.5 |
| 商品貨號 |
B164000 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with a combinatorial determinant of HLA A,B,C. The antibody cross-reacts with cells of various primates. The cells do poorly if hypoxanthine and thymidine are omitted from the medium tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against HLA A, B, C |
| Cellular Products |
immunoglobulin; monoclonal antibody; against HLA A, B, C |
| Comments |
Animals were immunized with papain solubilized HLA A2,B7. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with a combinatorial determinant of HLA A,B,C. The antibody cross-reacts with cells of various primates. The cells do poorly if hypoxanthine and thymidine are omitted from the medium tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Culture Medium: RPMI medium 1640 with 10(-4) M hypoxanthine, 1.6 x 10(-5) M
thymidine, 90%; fetal bovine serum, 10%. The cells do poorly if hypoxanthine
and thymidine are omitted. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
P Parham |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Brodsky FM, Parham P. Monomorphic anti-HLA-A,B,C monoclonal antibodies detecting molecular subunits and combinatorial determinants. J. Immunol. 128: 129-135, 1982. PubMed: 6172474
Brodsky FM, et al. Monoclonal antibodies for analysis of the HLA system. Immunol. Rev. 47: 3-61, 1979. PubMed: 95015
|
