| 產品名稱 | BCL1 clone 5B1b |
|---|---|
| 商品貨號 | B164015 |
| Organism | Mus musculus, mouse |
| Cell Type | B lymphocyte |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | leukemia; lymphoma |
| Gender | female |
| Strain | BALB/c |
| Applications | The cells can be activated by LPS to produce IgM and can be used as a target for B cell growth factor (BCGF) assays.
|
| Images |  |
| Clinical Data | female |
| Genes Expressed | immunoglobulin (surface, sIg +); monoclonal antibody |
| Cellular Products | immunoglobulin (surface, sIg +); monoclonal antibody |
| Comments | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium | RPMI 1640 medium with 2 mM L-glutamine and 0.05 mM 2-mercaptoethanol, 85%; fetal bovine serum, 15%
|
| Subculturing | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 days Subcultures are prepared by scraping. Dislodge cells in the spent medium, aspirate, add fresh medium and dispense into new flasks. |
| Isotype | IgD; IgM; lambda light chain |
| Name of Depositor | S Strober |
| Deposited As | Mus musculus |
| References | Gronowicz ES, et al. An in vitro line of the B cell tumor BCL1 can be activated by LPS to secrete IgM1. J. Immunol. 125: 976-980, 1980. PubMed: 6157730 Thoman ML, Weigle WO. Identification and partial purification of a B cell growth-promoting material utilizing the BCL1 cell line. J. Immunol. 130: 233-236, 1983. PubMed: 6600179 Strober S, et al. Immunobiology of a spontaneous murine B cell leukemia (BCL1). Immunol. Rev. 48: 169-195, 1979. PubMed: 121100 Slavin S, Strober S. Spontaneous murine B-cell leukaemia. Nature 272: 624-626, 1978. PubMed: 306069 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
