| 產品名稱 | BHK21-pcDNA3.1-HC |
|---|---|
| 商品貨號 | B164034 |
| Organism | Mesocricetus auratus, hamster, Syrian golden |
| Tissue | kidney |
| Cell Type | fibroblast |
| Product Format | frozen |
| Morphology | fibroblast |
| Culture Properties | adherent |
| Biosafety Level | 2 cells contain CMV and SV40 DNA viral particles
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | newborn |
| Applications | The cells secrete recombinant human erythropoietin in media, which can be detected by ELISA assay. This line was derived from ATCC PTA-419, which was derived from BHK-21(c-13) (ATCC CCL-10) [U.S. Pat. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | This line was derived from ATCC PTA-419, which was derived from BHK-21(c-13) (ATCC CCL-10) [U.S. Pat. 6,376,218]. The line was transfected by electroporation using the plasmid vector pcDNA3.1 containing a cDNA fragment encoding human erythropoietin. The vector contains cytomegalovirus (CMV) and SV40 viral DNA sequences and the neomycin resistance gene. The cells secrete recombinant human erythropoietin in media, which can be detected by ELISA assay. |
| Genes Expressed | recombinant human erythropoietin |
| Cellular Products | recombinant human erythropoietin (Hsu LW, Chang SC. Expression system for producing recombinant human erythropoietin, method for purifying secreted human erythropoietin and uses thereof. US Patent 6,376,218 dated Apr 28 2002) |
| Comments | This line was derived from ATCC PTA-419, which was derived from BHK-21(c-13) (ATCC CCL-10) [U.S. Pat. 6,376,218]. The line was transfected by electroporation using the plasmid vector pcDNA3.1 containing a cDNA fragment encoding human erythropoietin. The vector contains cytomegalovirus (CMV) and SV40 viral DNA sequences and the neomycin resistance gene. The cells secrete recombinant human erythropoietin in media, which can be detected by ELISA assay. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 0.4 mg/ml G-418; fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:8 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation | Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor | L Hsu, SC Chang |
| References | Hsu LW, Chang SC. Expression system for producing recombinant human erythropoietin, method for purifying secreted human erythropoietin and uses thereof. US Patent 6,376,218 dated Apr 28 2002 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
