| 產品名稱 |
BW5147.G.1.4.OUAR.1 |
| 商品貨號 |
B164062 |
| Organism |
Mus musculus, mouse |
| Cell Type |
T lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
lymphoma |
| Strain |
AKR/J |
| Applications |
They can be used to create T cell hybridomas. Tested and found negative for ectromelia virus (mousepox). |
| Antigen Expression |
H-2k |
| Genes Expressed |
H-2k |
| Comments |
The cells are ouabain resistant. They can be used to create T cell hybridomas. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; horse serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 3 X 10 exp4 viable cells/ml. Subculture every 3 to 4 days. |
| Name of Depositor |
R Hyman |
| Deposited As |
Mus musculus |
| References |
Hyman R, Stallings V. Complementation patterns of Thy-1 variants and evidence that antigen loss variants "pre-exist" in the parental population. J. Natl. Cancer Inst. 52: 429-436, 1974. PubMed: 4544550
|
