| 產品名稱 |
C166-GFP |
| 商品貨號 |
B164073 |
| Organism |
Mus musculus, mouse |
| Tissue |
Yolk sac |
| Cell Type |
endothelial |
| Product Format |
frozen |
| Morphology |
endothelial |
| Culture Properties |
adherent |
| Biosafety Level |
2
[Cells contain SV40 and HSV viral sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
12 day embryo |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
The C166-GFP cell line was derived from the C166 cell line (ATCC CRL-2581) by transfection with a plasmid reporter vector, pEGFP-N1, encoding enhanced green fluorescent protein (GFP). The vector was obtained from Clontech, Palo Alto, CA (#6085-1).
The C166 cell line was established from cells from F1 embryos obtained by mating a female NMRI/GSF mouse with a male CD-1 mouse that was transgenic for the human fes (fps/fes) proto-oncogene.
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| Comments |
The vector contains cytomegalovirus (CMV), SV40 and Herpes simplex virus (HSV) viral DNA sequences and the neomycin resistance gene.
These mice express multiple copies of an activated allele of the human fes (fps/fes) proto-oncogene and display hypervascularity progressing to multifocal hemangiomas.
For additional details see C166 (ATCC CRL-2581).
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| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002.
To make the complete growth medium, add the following components to the base medium:
0.2 mg/ml G -418
fetal bovine serum to a final concentration of 10%
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| Subculturing |
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze Medium: Complete growth medium 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| Name of Depositor |
R Auerbach |
| Deposited As |
mouse |
| References |
Wang SJ, et al. Isolation and propagation of yolk-sac-derived endothelial cells from a hypervascular transgenic mouse expressing a gain-of-function fps/fes proto-oncogene. In Vitro Cell. Dev. Biol. Anim. 32: 292-299, 1996. PubMed: 8792159
Lu LS, et al. In vitro and in vivo differentiation into B cells, T cells, and myeloid cells of primitive yolk sac hematopoietic precursor cells expanded > 100-fold by coculture with a clonal yolk sac endothelial cell line. Proc. Natl. Acad. Sci. USA 93: 14782-14787, 1996. PubMed: 8962132
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