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C305

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編號:B164082

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產品名稱	C305
商品貨號	B164082
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphoblast
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	It is useful for studying the T cell receptor signaling pathway using the Jurkat cell model. The antibody is specific for the beta chain of the T cell antigen receptor (TCR) on Jurkat cells.
Storage Conditions	liquid nitrogen vapor phase
Derivation	Animals were immunized with cells from the human T cell line, Jurkat, E6-1 (ATCC TIB-152). Spleen cells were fused with Sp2/0 myeloma cells. The antibody is specific for the beta chain of the T cell antigen receptor (TCR) on Jurkat cells. It is useful for studying the T cell receptor signaling pathway using the Jurkat cell model.
Genes Expressed	immunoglobulin; monoclonal antibody; against the beta chain of the T cell antigen receptor on Jurkat cells
Cellular Products	immunoglobulin; monoclonal antibody; against the beta chain of the T cell antigen receptor on Jurkat cells
Comments	Animals were immunized with cells from the human T cell line, Jurkat, E6-1 (ATCC TIB-152). Spleen cells were fused with Sp2/0 myeloma cells. The antibody is specific for the beta chain of the T cell antigen receptor (TCR) on Jurkat cells. It is useful for studying the T cell receptor signaling pathway using the Jurkat cell model.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing	Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension between 1 x 10⁵ and 2 x 10⁵ viable cells/mL. Maintain cultures at a cell concentration between 1 x 10⁵ and 1 x 10⁶cells/mL. Do not allow the cell concentration to exceed 1 x 10⁶ cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days (as cell density increases).
Cryopreservation	Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Isotype	IgM kappa
Name of Depositor	A Weiss
Deposited As	mouse (B cell); mouse (myeloma)
References	Weiss A, Stobo JD. Requirement for the coexpression of T3 and the T cell antigen receptor on a malignant human T cell line. J. Exp. Med. 160: 1284-1299, 1984. PubMed: 6208306 Goldsmith MA, Weiss A. Early signal transduction by the antigen receptor without commitment to T cell activation. Science 240: 1029-1031, 1988. PubMed: 3259335 Wu J, et al. Vav and SLP-76 interact and functionally cooperate in IL-2 gene activation. Immunity 4: 593-602, 1996. PubMed: 8673706 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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