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C3H/MCA clone 16

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編號:B164090

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產(chǎn)品名稱	C3H/MCA clone 16
商品貨號	B164090
Organism	Mus musculus, mouse
Tissue	embryo
Product Format	frozen
Morphology	fibroblast
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age	embryo
Strain	C3H
Applications	The line was derived by treating C3H/10T1/2 clone 8 cells with 3-methylcholanthrene. Tested and found negative for ectromelia virus (mousepox).
Derivation	The line was derived by treating C3H/10T1/2 clone 8 cells with 3-methylcholanthrene.
Antigen Expression	H-2k
Genes Expressed	H-2k
Tumorigenic	Yes
Effects	Yes, in C3H mice
Comments	The line was derived by treating C3H/10T1/2 clone 8 cells with 3-methylcholanthrene. Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium	The base medium for this cell line is Eagle's Basal medium with 2 mM L-glutamine , 1.5 g/L sodium bicarbonate and Earle's BSS. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
Subculturing	Medium Renewal: 2 to 3 times per week Cultures are prepared using 0.1% trypsin in calcium and magnesium free phosphate buffered saline. New cultures are seeded at 3 X 10 exp3 viable cells/sq cm. Subculture every 6 to 7 days.
Name of Depositor	C Heidelberger
Deposited As	Mus musculus
References	Reznikoff CA, et al. Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division. Cancer Res. 33: 3239-3249, 1973. PubMed: 4796800 Terzaghi M, Little JB. Repair of potentially lethal radiation damage in mammalian cells is associated with enhancement of malignant transformation. Nature 253: 548-549, 1975. PubMed: 1167940 Mondal S, Heidelberger C. Transformation of C3H/10T1/2 CL8 mouse embryo fibroblasts by ultraviolet irradiation and a phorbol ester. Nature 260: 710-711, 1976. PubMed: 1264242 Rapp UR, et al. Endogenous oncornaviruses in chemically induced transformation. I. Transformation independent of virus production. Virology 65: 392-409, 1975. PubMed: 165619 Chan GL, Little JB. Induction of oncogenic transformation in vitro by ultraviolet light. Nature 264: 442-444, 1976. PubMed: 1004574 Reznikoff CA, et al. Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division. Cancer Res. 33: 3231-3238, 1973. PubMed: 4357355

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