| 產品名稱 |
C5/MJ |
| 商品貨號 |
B164095 |
| Organism |
Homo sapiens, human |
| Tissue |
cord blood |
| Cell Type |
T lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
2 [Cells contain Retrovirus]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Leukemia |
| Age |
newborn |
| Gender |
male |
| Applications |
The cells produce Human T cell Leukemia virus (HTLV-I) and HTLV related antigens. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
This line was established by Popovic et al. in 1983 from neonatal cord blood by co-cultivation with an HTLV-I producing cell line, MJ (see ATCC CRL-8294). |
| Clinical Data |
male
neonatal |
| Comments |
The cells express a cell surface glycoprotein of 61000 daltons (45000 daltons when not glycosylated) that is characteristic of HTLV infection. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at about 2 to 4 x 105 cells/mL and maintain between 4 x 105 and 1 x 106 cells/mL.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week |
| Cryopreservation |
Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions |
Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| STR Profile |
Amelogenin: X,Y CSF1PO: 9,11 D13S317: 12 D16S539: 8,11 D5S818: 13 D7S820: 11,12 THO1: 6,9 TPOX: 8 vWA: 16,17 |
| Name of Depositor |
President and Fellows of Harvard College |
| Deposited As |
Homo sapiens |
| U.S. Patent Number |
|
| Year of Origin |
1983 |
| References |
Essex ME, Lee TH. Method and products for detection of human T cell leukemia virus. US Patent 4,743,678 dated May 10 1988
Popovic M, et al. Isolation and transmission of human retrovirus (human t-cell leukemia virus). Science 219: 856-859, 1983. PubMed: 6600519
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
|
