| 產(chǎn)品名稱(chēng) |
C5B7 |
| 商品貨號(hào) |
B164097 |
| Organism |
Homo sapiens, human |
| Tissue |
spleen |
| Cell Type |
B lymphocyte; Epstein-Barr virus (EBV) transforme |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
2 Cells Contain HERPESVIRUS
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody is specific for the lipopolysaccharide of the Fisher immunotype 1 of P. aeruginosa. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
B cells obtained from a patient with cystic fibrosis (whose serum contained above normal titer to P. aeruginosa) were transformed with Epstein-Barr virus (EBV). |
| Clinical Data |
B cells obtained from a patient with cystic fibrosis (whose serum contained above normal titer to P. aeruginosa) were transformed with Epstein-Barr virus (EBV). |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against Pseudomonas aeruginosa lipopolysaccharide (LPS) of Fisher immunotype 1 (IATS type 6) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against Pseudomonas aeruginosa lipopolysaccharide (LPS) of Fisher immunotype 1 (IATS type 6) |
| Comments |
B cells obtained from a patient with cystic fibrosis (whose serum contained above normal titer to P. aeruginosa) were transformed with Epstein-Barr virus (EBV). The antibody is specific for the lipopolysaccharide of the Fisher immunotype 1 of P. aeruginosa. |
| Complete Growth Medium |
Iscove's modified Dulbecco's medium with 2 mM L-glutamine, 85%; heat-inactivated fetal bovine serum, 15%
|
| Subculturing |
Medium Renewal: Every 3 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Isotype |
IgM |
| Population Doubling Time |
24 to 36 hrs |
| Name of Depositor |
Miles Inc., Genetics Systems Corporation |
| Deposited As |
Homo sapiens |
| U.S. Patent Number |
|
| References |
Siadak AW, Lostrom ME. Human monoclonal antibodies to serotypic lipopolysaccharide determinants on Gram-negative bacteria and their production. US Patent 4,834,975 dated May 30 1989
|
