| 產品名稱 |
CBL-1 |
| 商品貨號 |
B164124 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Leukemia |
| Applications |
The antibody binds to and blocks the action of the growth factor. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against a 15000 dalton human autocrine growth factor of tumor cells and activated lymphocytes |
| Cellular Products |
immunoglobulin; monoclonal antibody; against a 15000 dalton human autocrine growth factor of tumor cells and activated lymphocytes |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in pristane primed BALB/cJ mice |
| Comments |
Animals were immunized with human acute leukemia cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody binds to and blocks the action of the growth factor. |
| Complete Growth Medium |
CULTURE MEDIUM: RPMI medium 1640, 90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgM |
| Name of Depositor |
R Billing, PI Terasaki, Regents of the University of California |
| U.S. Patent Number |
|
| References |
Billing RJ. Monoclonal antibodies to an autocrine growth factor antigen that binds to activated lymphocytes and cancer cells. US Patent 5,330,896 dated Jul 19 1994
|
