| 產品名稱 |
CC57 |
| 商品貨號 |
B164138 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody is specific for a 90000 dalton molecule expressed on the surface of bovine B cells. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Spleen cells were fused with NS1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against bovine WC4 B cell antigen (BoWC4) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against bovine WC4 B cell antigen (BoWC4) |
| Comments |
Animals were immunized with bovine mesenteric lymph node cells. Spleen cells were fused with NS1 myeloma cells. The antibody is specific for a 90000 dalton molecule expressed on the surface of bovine B cells. This antibody was included in the panel of the International Workshop on Bovine, Sheep and Goat Leukocyte Differentiation Antigens in 1989 and 1992. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium.
Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) cells/ml. Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype |
mouse IgG1 |
| Name of Depositor |
CJ Howard |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Hein WR, et al. Epitopes of the T19 lymphocyte surface antigen are extensively conserved in ruminants. Vet. Immunol. Immunopathol. 27: 173-181, 1991. PubMed: 1708546
. . Vet. Immunol. Immunopathol. 32: 1993..
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