| 產品名稱 |
CCD-1070Sk |
| 商品貨號 |
B164154 |
| Organism |
Homo sapiens, human |
| Tissue |
skin |
| Cell Type |
fibroblast |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
normal |
| Age |
newborn |
| Gender |
male |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
The line was established from skin taken from normal foreskin. |
| Clinical Data |
male
newborn |
| Comments |
Cells senesce after approximately 36 population doublings. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37°C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C |
| Population Doubling Capacity |
Cells senesce after approximately 36 population doublings. |
