| 產(chǎn)品名稱 |
CCF-STTG1 |
| 商品貨號 |
B164215 |
| Organism |
Homo sapiens, human |
| Tissue |
brain |
| Product Format |
frozen |
| Morphology |
astrocytic |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
grade IV, astrocytoma |
| Age |
68 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
This human cell line contained large numbers of tetra-, hexa-, and higher-ploid cells (2s populations). The modal (s) cell population which occurred in 32% of cells had the pseudodiploid karyotype, 46,XX,-3,-18,del(7) (q21.12;q22.3), ?t(3q?18q). The rate of 2s cells was 42%. Two marker chromosomes, del(7) (q21.12;q22.3) and ?t(3q?18q), were present in all s metaphases, but ?t(3q?18q) was absent in 2s cells. DMs were found in some s cells whereas they were seen in the majority of 2s cells. HSR chromosomes were not found. In s metaphases, both N3 and N18 had only single copy, and the X chromosome was paired. |
| Derivation |
The CCF-STTG1 cell line was established from a specimen of Grade IV astrocytoma from a 68-year-old Caucasian female by mincing and culturing in RPMI 1640 with 10% FBS. |
| Clinical Data |
68 years
Caucasian
female
|
| Antigen Expression |
HLA-DR; Homo sapiens, expressed |
| Comments |
The cells exhibit strong perinuclear acid phosphatase activity and glial fibrillary acidic protein is present in 70% to 80% of the cells. HLA DR is expressed on 20% to 30% of the cells after 48 hours in culture. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
- Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
-
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 3 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 12 D13S317: 11,13 D16S539: 11,12 D5S818: 12,13 D7S820: 10,11 THO1: 7,8 TPOX: 8,11 vWA: 17 |
| Name of Depositor |
BP Barna |
| Deposited As |
Homo sapiens |
| References |
. Experimental allergic encephalitis. New York: Liss; 1984.
. . In Vitro 19: 275, 1983.
. . Fed. Proc. 43: 781, 1984.
Barna BP, et al. Enhanced DNA synthesis of human glial cells exposed to human leukocyte products. J. Neuroimmunol. 10: 151-158, 1985. PubMed: 3877740
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