| 產品名稱 |
CCRF-HSB-2 [CCRF HSB-2, HSB-2] |
| 商品貨號 |
B164218 |
| Organism |
Homo sapiens, human |
| Tissue |
peripheral blood |
| Cell Type |
T lymphoblast |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
acute lymphoblastic leukemia |
| Age |
11.5 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Derived from the same buffy coat preparation as CCL-120 (CCRF-SB) by serially transplanting into newborn syrian hamsters. |
| Clinical Data |
11.5 years Caucasian male |
| Antigen Expression |
HLA A1, A2, B12, B17, Cw2; CD5 (78%), CD7 (96%) |
| Tumorigenic |
Yes, in Syrian hamsters |
| Effects |
Yes, in Syrian hamsters |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 x 105 viable cells/mL.
A maximum of 2 to 3 x 106 viable cells/mL is obtainable.
Medium Renewal: Every 2 to 3 days
|
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 10,12 D13S317: 10,12 D16S539: 9,13 D5S818: 11,12 D7S820: 11,12 THO1: 9,10 TPOX: 8 vWA: 18,19 |
| Isoenzymes |
G6PD, B |
| Name of Depositor |
GE Foley |
| Deposited As |
Homo sapiens |
| References |
Foley GE et al. M.D. Anderson Hospital and Tumor Inst. Monograph 21, 1967.
Adams RA, et al. Direct implantation and serial transplantation of human acute lymphoblastic leukemia in hamsters, SB-2. Cancer Res. 28: 1121-1125, 1968. PubMed: 4872716
Proc. Am. Assoc. Cancer Res. 8: 1, 1967.
|
| Cross References |
Nucleotide (GenBank) :
NM_004429
Homo sapiens ephrin-B1 (EFNB1), mRNA.
Nucleotide (GenBank) :
U09303
Human T cell leukemia LERK-2 (EPLG2) mRNA, complete cds.
Nucleotide (GenBank) :
NM_004429
Homo sapiens ephrin-B1 (EFNB1), mRNA.
Nucleotide (GenBank) :
U09303
Human T cell leukemia LERK-2 (EPLG2) mRNA, complete cds.
|
