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CO 88BV59-1

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編號:B164279

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產品名稱	CO 88BV59-1
商品貨號	B164279
Organism	Homo sapiens, human
Tissue	peripheral blood
Cell Type	B lymphocyte; Epstein-Barr virus (EBV) transformed
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	2 [Herpes virus, cells contain Epstein-Barr virus (EBV)] Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	cancer
Applications	Peripheral blood mononuclear cells (PBMNC) were transformed with Epstein-Barr virus (EBV) derived from the B95-8 marmoset cell line. CO 88BV59-1 cells produce monoclonal antibody 88BV59 that has been shown to recognize colon tumor associated antigen (CTAA) 16.88, also referred to as CTA #1. The CO 88BV59 cell line was fused with a human-mouse heteromyeloma to produce a cell line designated Co88BV59H21-2 (ATCC CRL-11538). Co88BV59H21-2 was then exposed to EBV under conditions suitable for transformation to produce Co88BV59H21-2V67-66 (ATCC CRL-11539).
Storage Conditions	liquid nitrogen vapor phase
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Karyotype	diploid
Derivation	This transformed cell line was derived from B-cells of a patient undergoing surgical resection of colorectal cancer. The patient was actively immunized with autologous tumor antigen. Peripheral blood mononuclear cells (PBMNC) were transformed with Epstein-Barr virus (EBV) derived from the B95-8 marmoset cell line. The CO 88BV59 cell line was fused with a human-mouse heteromyeloma to produce a cell line designated Co88BV59H21-2 (ATCC CRL-11538).
Genes Expressed	immunoglobulin; monoclonal antibody; against colon tumor associated antigen (CTAA) 16.88.
Cellular Products	immunoglobulin; monoclonal antibody; against colon tumor associated antigen (CTAA) 16.88. (Hanna MG Jr., et al. Tumor associated monoclonal antibodies. US Patent 5,474,755 dated Dec 12 1995)
Comments	The patient was actively immunized with autologous tumor antigen. Peripheral blood mononuclear cells (PBMNC) were transformed with Epstein-Barr virus (EBV) derived from the B95-8 marmoset cell line. CO 88BV59-1 cells produce monoclonal antibody 88BV59 that has been shown to recognize colon tumor associated antigen (CTAA) 16.88, also referred to as CTA #1. Both the 88BV59 antibody and the 16-88 antibody recognize the same tumor associated antigen, but react with different epitopes on that antigen. The 16-88 antibody (LiCO 16-88) is available as ATCC HB-8495. The CO 88BV59 cell line was fused with a human-mouse heteromyeloma to produce a cell line designated Co88BV59H21-2 (ATCC CRL-11538). Co88BV59H21-2 was then exposed to EBV under conditions suitable for transformation to produce Co88BV59H21-2V67-66 (ATCC CRL-11539).
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium (IMDM; ATCC 30-2005). To make the complete growth medium, add the following components to the base medium: fetal bovine serum (FBS; ATCC 30-2020) to a final concentration of 20%.
Subculturing	Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 8 x 10⁵ viable cells/mL.Maintain cell density between 5 x 10⁵and 2 x 10⁶ viable cells/mL. Medium Renewal: Every 2 to 3 days
Cryopreservation	Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C Atmosphere: 5% Carbon dioxide (CO₂)
STR Profile	Amelogenin: X,Y CSF1PO: 10,11 D13S317: 11 D16S539: 9,11 D5S818: 10,12 D7S820: 8,10 THO1: 6,9.3 TPOX: 8,11 vWA: 15,16
Isotype	IgG3; kappa light chain
Name of Depositor	Organon Teknika Biotech Research Institute
Deposited As	human
U.S. Patent Number	5,474,755
References	Hanna M Jr., et al. Tumor associated monocoloal antibodies derived from human B-cell line. US Patent 4,997,762 dated Mar 5 1991 Hanna MG Jr., et al. Tumor associated monoclonal antibodies. US Patent 5,474,755 dated Dec 12 1995 Butler SM, et al. Tumor associated epitope. US Patent 5,951,985 dated Sep 14 1999 De Jager R, et al. Current status of cancer immunodetection with radiolabeled human monoclonal antibodies. Semin. Nucl. Med. 23: 165-179, 1993. PubMed: 8511602 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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