| 產(chǎn)品名稱 |
CVC.7 |
| 商品貨號 |
B164306 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
This antibody has the same specificity as CVC.1 (ATCC TIB-135). Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against bovine brain clathrin (light chain, 36000 dalton) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against bovine brain clathrin (light chain, 36000 dalton) |
| Comments |
Animals were immunized with bovine brain clathrin. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. This antibody has the same specificity as CVC.1 (ATCC TIB-135). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: 2 to 3 times per week Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype |
IgG2a |
| Name of Depositor |
FM Brodsky, P Parham |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Kirchhausen T, et al. Location and distribution of the light chains in clathrin trimers. Proc. Natl. Acad. Sci. USA 80: 2481-2485, 1983. PubMed: 6189124
Brodsky FM, Parham P. Polymorphism in clathrin light chains from different tissues. J. Mol. Biol. 167: 197-204, 1983. PubMed: 6864801
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